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klenow exo  (New England Biolabs)


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    New England Biolabs klenow exo
    Klenow Exo, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 312 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/klenow exo/product/New England Biolabs
    Average 95 stars, based on 312 article reviews
    klenow exo - by Bioz Stars, 2026-03
    95/100 stars

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    ( A ) Schematic diagrams depicting the polymerization catalyzed by DNA polymerase I, the ligation catalyzed by T4 DNA ligase and the digestion catalyzed by DNase I, along with comparisons of interfacial reaction efficiency when using AVS versus not using AVS. ( B ) Schematic diagram outlining the iEOS process on AVS. ( C ) Comparison of the stepwise yield, full-length yield, and deletion error rate with and without the use of AVS. ( D ) On the basis of NGS results, a synthetic step-by-step yield was determined through comparison with the target sequence (* P < 0.05; ** P ≤ 0.001; *** P ≤ 0.0001).

    Journal: Science Advances

    Article Title: Scalable acoustic virtual stirrer for enhanced interfacial enzymatic nucleic acid reactions

    doi: 10.1126/sciadv.adt6955

    Figure Lengend Snippet: ( A ) Schematic diagrams depicting the polymerization catalyzed by DNA polymerase I, the ligation catalyzed by T4 DNA ligase and the digestion catalyzed by DNase I, along with comparisons of interfacial reaction efficiency when using AVS versus not using AVS. ( B ) Schematic diagram outlining the iEOS process on AVS. ( C ) Comparison of the stepwise yield, full-length yield, and deletion error rate with and without the use of AVS. ( D ) On the basis of NGS results, a synthetic step-by-step yield was determined through comparison with the target sequence (* P < 0.05; ** P ≤ 0.001; *** P ≤ 0.0001).

    Article Snippet: Following this, a 10-μl polymerization reaction buffer was prepared by mixing Klenow fragment of DNA polymerase (1 U/μl M0210, NEB), 100 μM deoxycytidine triphosphate–Cy3, 5 mM deoxy-ribonucleoside triphosphate (dNTP) mix, 50 mM NaCl, 10 mM tris-HCl, 10 mM MgCl 2 , and 1 mM dithiothreitol (DTT) (pH 7.9).

    Techniques: Ligation, Comparison, Sequencing